ASSOCIATIONS OF SHORT AND MEDIUM CHAIN SATURATED FATTY ACIDS AND DAIRY WITH COGNITIVE FUNCTIONING IN THE BOSTON PUERTO RICAN HEALTH STUDY

Cognitive decline is a major public health concern. Evidence suggests that Hispanic/Latino adults, specifically Puerto Rican adults, in the US are at a higher risk of developing dementia or cognitive decline due to the high prevalence of risk factors, including cardiovascular disease and diabetes. Emerging evidence suggests that specific dietary fatty acids, short and medium-chain-length saturated fatty acids, that are found in dairy may be beneficial for cognitive function. However, there are limited observational studies examining the effects of SMCSFA and dairy on cognitive function in Hispanic/Latino adults. We examined the cross-sectional and prospective associations between SMCSFA and dairy consumption with cognitive function among Puerto Rican adults. Data were from the Boston Puerto Rican Health Study (57 yrs, 71% female), an ongoing prospective cohort study. Diet was assessed using a validated food frequency questionnaire. Dairy products included milk, cheese, yogurt, cream, and butter. Our primary exposures were the sum of SMCSFA(%TE), a dietary fatty acid pattern consistent with high SMCSFA, total dairy (s/d), regular & reduced fat dairy (s/d), and nonfat dairy products (s/d). A battery of neurocognitive tests was administered by trained staff in the language of preference. Global cognitive function score (GCS) was calculated as the mean z-scores of the individual tests. A subset of BPRHS participants returned for neurocognitive testing at 13-yr follow-up. We analyzed SMCSFA in a substitution analysis at the expense of trans fats and added sugar. Dairy models were adjusted for total energy, age, sex, physical activity, smoking status, and education. We also conducted a substation analysis of dairy at the expense of red and processed meats. Our cross-sectional analysis was conducted using multivariate linear regression. Our prospective analysis assessed the change over baseline, 2 and 13 years using a mixed effects model with time-covarying covariates. In the final sample, participants consumed 2.42 s/day of dairy and less than 1% of their diet was derived from SMCSFA. Most dairy was consumed through 2% and whole milk (37%). Most SMCSFA in the diet were derived from cheese (30%) and whole milk (22%). Butter was significantly related to GCS over 13 years of follow-up. There were no significant associations between SMCSFA, total dairy, regular & reduced fat dairy, or nonfat dairy with cognitive function cross-sectionally or prospectively. In this cohort of Puerto Rican adults, our findings suggest there were no associations between SMCSFA and dairy with cognitive function. Future prospective studies should examine this relationship in a similar population with larger sample sizes and over a longer duration

Characterization and in vitro assessment of seaweed bioactives with potential to reduce methane production

This study collates compositional analysis of seaweeds data with information generated from in vitro gas production assays in the presence and absence of seaweeds. The aim was to assess and rank 27 native northern European seaweeds as potential feed ingredients for use to reduce methane emissions from ruminants. It provides information for use in future in vivo dietary trials concerning feed manipulation strategies to reduce CH4 emissions efficiently from domestic ruminants based on dietary seaweed supplementation. The seaweeds H. siliquosa and A. nodosum belonging to phylum Phaeophyta displayed the highest concentration of phlorotannins and antioxidant activity among the macroalgae giving anti-methanogenic effect in vitro, while this explanation was not valid for the observed reduction in methane when supplementing with C. filum and L. digitata in this study. D. carnosa and C. tenuicorne belonging to phylum Rhodophyta had the highest protein content among the macroalgae that reduced methane production in vitro. There were no obvious explanation from the compositional analysis conducted in this study to the reduced methane production in vitro when supplementing with U. lactuca belonging to phylum Chlorophyta. The strongest and most complete methane inhibition in vitro was observed when supplementing with Asparagopsis taxiformis that was used as a positive control in this study

The incurable self: Negotiating social bonds and dis/connection with metastatic breast cancer

As the culture of silence that once surrounded cancer has gradually given way to greater public awareness, normative visions of what cancer survivorship should entail have proliferated. These visions emphasise positivity and perseverance in pursuit of cure. While these visions provide comfort to many, for people with metastatic cancer the emphasis on cure can undermine their sense of belonging to the broader collective of people living-with cancer. Drawing on semi-structured interviews with 38 Australian women living with metastatic breast cancer, we explore how incurable cancer inflects understandings of self and transforms interpersonal relationships. Extending ideas around biosociality and belonging, we explore the tenuousness of social bonds, revealing how (in)visibility, (in)authenticity and (in)validation circulate within the daily lives of women with metastatic breast cancer. We conceptualise accounts according to four social bonds: (1) threatened bonds where a relationship is strained by misunderstanding, (2) severed bonds where a relationship is ruptured due to misunderstanding, (3) attuned bonds whereby a relationship is based on shared identification, and (4) flexible social bonds when a relationship is based on mutual understanding. More broadly, we illustrate the persistence of normative visions of cancer survivorship and their enduring effects on those whom such visions exclude

The social meanings of choice in living-with advanced breast cancer

Individual choice is valorised as a core social value; yet the necessity and desirability of making choices takes on new significance for people living with incurable cancer who are required to make often difficult decisions about treatment, care and family life, amidst considerable vulnerability and precariousness. There has been comparatively little exploration of how choice is negotiated and made meaningful under the spectre of incurability and a contracted future. In this paper, drawing on multiple qualitative interviews with 38 women with metastatic breast cancer, we explore how they experience and give meaning to choice in relation to their health (and beyond) in their daily lives. Our analysis highlights that while exercising choice was sometimes a concealed or silent pursuit, choice was always a socially negotiated and temporally unfolding process, nested within relational and interpersonal dynamics. Choices were also often constrained, even foreclosed, due to situational and relational dynamics. Yet even in the absence of choice, the idea of choice-as-control was discursively embraced by women. We argue that greater attention is needed to the affective, temporal and economic dimensions of choice, and how treatment decisions are asymmetrically structured when considered within the normative context of cancer

Presence and Cybersickness in Virtual Reality Are Negatively Related: A Review

In order to take advantage of the potential offered by the medium of virtual reality (VR), it will be essential to develop an understanding of how to maximize the desirable experience of “presence” in a virtual space (“being there”), and how to minimize the undesirable feeling of “cybersickness” (a constellation of discomfort symptoms experienced in VR). Although there have been frequent reports of a possible link between the observer’s sense of presence and the experience of bodily discomfort in VR, the amount of literature that discusses the nature of the relationship is limited. Recent research has underlined the possibility that these variables have shared causes, and that both factors may be manipulated with a single approach. This review paper summarizes the concepts of presence and cybersickness and highlights the strengths and gaps in our understanding about their relationship. We review studies that have measured the association between presence and cybersickness, and conclude that the balance of evidence favors a negative relationship between the two factors which is driven principally by sensory integration processes. We also discuss how system immersiveness might play a role in modulating both presence and cybersickness. However, we identify a serious absence of high-powered studies that aim to reveal the nature of this relationship. Based on this evidence we propose recommendations for future studies investigating presence, cybersickness, and other related factors

Mycobacterium bovis genomics reveals transmission of infection between cattle and deer in Ireland

Control of bovine tuberculosis (bTB), caused by Mycobacterium bovis, in the Republic of Ireland costs €84 million each year. Badgers are recognised as being a wildlife source for M. bovis infection of cattle. Deer are thought to act as spillover hosts for infection; however, population density is recognised as an important driver in shifting their epidemiological role, and deer populations across the country have been increasing in density and range. County Wicklow represents one specific area in the Republic of Ireland that has had consistently high bTB prevalence for over a decade, despite control operations in both cattle and badgers. The area is also known to have a high density of deer. Our research used whole genome sequencing of M. bovis sourced from infected cattle, deer, and badgers in County Wicklow to evaluate whether the epidemiological role of deer could have shifted from spillover host to source. Our analyses reveal that cattle and deer share highly similar M. bovis strains suggesting that transmission between these species is occurring in the area. In addition, the high level of diversity observed in the sampled deer population suggests deer may be acting as a source of infection for local cattle populations. These findings have important implications for the control and ultimate eradication of bTB in Ireland

Single-Cell Sequencing of Developing Human Gut Reveals Transcriptional Links to Childhood Crohn's Disease.

Human gut development requires the orchestrated interaction of differentiating cell types. Here, we generate an in-depth single-cell map of the developing human intestine at 6-10 weeks post-conception. Our analysis reveals the transcriptional profile of cycling epithelial precursor cells; distinct from LGR5-expressing cells. We propose that these cells may contribute to differentiated cell subsets via the generation of LGR5-expressing stem cells and receive signals from surrounding mesenchymal cells. Furthermore, we draw parallels between the transcriptomes of ex vivo tissues and in vitro fetal organoids, revealing the maturation of organoid cultures in a dish. Lastly, we compare scRNA-seq profiles from pediatric Crohn's disease epithelium alongside matched healthy controls to reveal disease-associated changes in the epithelial composition. Contrasting these with the fetal profiles reveals the re-activation of fetal transcription factors in Crohn's disease. Our study provides a resource available at www.gutcellatlas.org, and underscores the importance of unraveling fetal development in understanding disease

Personhood, belonging, affect and affliction

What does migrancy mean for personhood, and how does this flow through caring relations? Drawing on life history interviews and photo elicitation with 43 people who identify as migrants and live with cancer, here we argue for the significance of recognising complex personhood as it inflects illness and care. Drawing on social science theory around temporalities, moralities and belonging, we assemble a series of cross-cutting themes at the intersection of personhood and care; relations that transcend cultural origins yet are vividly illustrated in relation to migrant pasts. In seeking a multidimensional view of personhood, we attend to the intersecting layers of complexity that make up care in this context vis-a-vis an emphasis on forms of difference, vulnerability and otherness. In this way, we develop an approach to personhood and care that broadens the lens on migrancy and cancer, but also, one that speaks to the importance of recognition of complexity and how it shapes care more generally

Evidence for local and international spread of Mycobacterium avium subspecies paratuberculosis through whole genome sequencing of isolates from the island of Ireland

Publication history: Accepted - 1 April 2022; Published online - 5 April 2022We describe application of whole genome sequencing (WGS) to a collection of 197 Mycobacterium avium subsp paratuberculosis (MAP) isolates gathered from 122 cattle herds across 27 counties of the island of Ireland. We compare WGS to MAP diversity quantified using mycobacterial interspersed random unit – variable number tandem repeats (MIRU-VNTR). While MIRU-VNTR showed only two major types, WGS could split the 197 isolates into eight major groups. We also found six isolates corresponding to INMV 13, a novel MIRU-VNTR type for Ireland. Evidence for dispersal of MAP across Ireland via cattle movement could be discerned from the data, with mixed infections present in several herds. Furthermore, comparisons of MAP WGS data from Ireland to data from Great Britain and continental Europe revealed many instances of close genetic similarity and hence evidence for international transmission of infection. BEAST MASCOT structured coalescent analyses, with relaxed and strict molecular clocks, estimated the substitution rate to be 0.10–0.13 SNPs/site/year and disclosed greater transitions per lineage per year from Europe to Ireland, indicating transmission into Ireland. Our work therefore reveals new insight into the seeding of MAP infection across Ireland, highlighting how WGS can inform policy formulation to ultimately control MAP transmission at local, national and international scales.We acknowledge funding from the Department of Agriculture, Food and Marine awards 15/S/651 (‘NexusMAP’) and 2019R404 (’BTBGe- nIE’)